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IBM 000-965 Study Materials with Actual 000-965 Exam

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Comments (79) Posted to interest 03/11/2009 Edit

EA loosens criticized activation policy on Spore

Faced with growing criticism about the way its newly released game Spore (SPORE-RELOADED) is activated on computers, gaming publishing giant Electronic Arts did a little retooling of its own. EA has increased the number of computers that can be loaded with the game to five from three, despite earlier precautions with its digital rights management (DRM) policy intended to reduce piracy of its copyrighted software. Spore, released two weeks ago featuring unlikely creatures that can be tailored to the user’s liking, has altered other DRM limitations embedded in the software, the company announced. Frank Gibeau, EA Games Label president, said in a statement: We’ve received complaints from a lot of customers who we recognize and respect. And while it’s easy to discount the noise from those who only want to post or transfer thousands of copies of the game on the Internet, I believe we need to adapt our policy to accommodate our legitimate consumers. EA announced it will not only increase the number of computers that users can load one copy of Spore onto, but will also offer ways in which users can receive additional activations of the gaming software if warranted. The game publisher also plans to fast-track its development efforts on creating a system that will allow consumers to de-authorize machines and transfer authorizations to new computers. EA said it believed at the time it created Spore that its DRM policy would not present any problems. The company noted that 75 percent of its customers tended to use only one computer for running their games and that less than 1 percent of its users relied on three or more computers. It also added that it told consumers that they could receive more than three activations if warranted by calling into the company’s customer service. Source: Cnet

Comments (216) Posted to interest 09/22/2008 Edit

Virginia Supreme Court: Spam is fine!

The Virginia Supreme Court has decided that the state’s anti-spam law is unconstitutional and freed a man once considered one of the world’s most prolific spammers. The court unanimously agreed that the law violates free speech because it does not just restrict commercial e-mails it blocks all unsolicited messages too. Other states have anti-spam laws but those laws apply only to commercial e-mail. Justice G. Steven Agee said that the Virginia law was silly because prohibits the anonymous transmission of all unsolicited bulk e-mails, including those containing political, religious or other speech protected by the First Amendment.

He pointed out that the famous Federalist Papers urging ratification of the Constitution would be labelled Spam under this law if they had been sent as email. The writers Alexander Hamilton, James Madison and John Jay would have gone to jail as spammers, rather than the French-backed terrorists against the lawful government, that they were. The move means that Jeremy Jaynes, the first person in the U.S. to be convicted of sending spam, will be freed from the charge. He sent up to 10 million e-mails a day from his home in Raleigh. In 2004, Jaynes was sentenced to nine years in the Clink. Fortunately for those who don’t like spam he remains inside jail because he is also serving time in federal prison for a securities fraud conviction. This has nothing to do with spam, where he was just expressing free speech, apparently.

Source: Inquirer

Comments (6) Posted to interest 09/16/2008 Edit

Google dominates the market with 60% share

Comscore as well as Nielsen Netratings estimated Google’s U.S. search engine market at more than 60% in July. While the market research firms have come up with substantially different numbers for their July report, both agree that Google now runs more than three times the numbers of searches of its closest rival and more than twice the searches of Yahoo and Microsoft combined. Comscore and Nielsen Netratings posted market share numbers for Google at greater than 60% for the second time this year. However, while the April result could have been considered as a blip, the July result fits the pattern of Google chipping away market share from its rivals, bit by bit.

Nielsen Netratings estimates that Google was used for 60.2% or more than 4.8 billion of 8.0 billion searches started by U.S. users during the month of July. Comcast came up with a 61.9% share for Google and 7.3 billion searches of a total of a total of 11.8 billion. Both Yahoo and Microsoft keep losing ground: Nielsen puts the two companies at 17.4% and 11.9%, Comscore at 20.5% and 8.9%. Google’s market share gains appear to have accelerated in 2008, with much stronger growth than in previous years. According to Nielsen Netratings, Google had a market share of 46.2% in July 2005, 49.2% in July 2006 and 53.3% in July 2007. Over the past year, Google was able to increase its market share by almost seven percentage points – more than in any other 12-month period before.

Source: TG Daily

Comments (8) Posted to interest 09/02/2008 Edit

Diablo 2 CD-Key Issues

Are you seeing the message "Your CD key is disabled from realm play"?

• If you are receiving the error message, “Your CD key is disabled from realm play.” when trying to join or create a realm game, this indicates the CD-Key installed has been temporarily or permanently banned from playing realm games on Battle.net. Even though the CD-Key is restricted from playing realm games, you are still able to play Open Battle.net games.
• Diablo II CD Key are regularly banned and accounts are closed due to hacking on Battle.net. In the case of the CD-Keys that were banned, at least one account tied to each CD-Key was identified as having used such a program while playing on Battle.net. First time offenders are banned from realm play for one month and repeat offenders are banned from realm play permanently. Users who are unsure if their CD-Key was temporarily or permanently banned will have to wait one month from the time the CD-Key was banned to determine the status.

• If you get the message that your "CD-Key in use by [Your Name]", then you should only have to wait 15-30 minutes before reconnecting to Battle.net. This is usually caused by a recent disconnection from Battle.net. The [name] listed in this error message is not a Battle.net account name - it is the name entered when the game was installed. If this [name] is the one you entered, then Battle.net has not yet recognized that you have logged off of Battle.net. Also remember that each CD-Key can only be logged into Battle.net once, so be sure you have only installed your Diablo 2 CD Key on one machine.
• If the problem persists, please try restarting your computer or powering your modem/router off and then back on. If you are unsure how to do this; please check the hardware documentation or contact the manufacturer directly for assistance.
• If you have waited 30 minutes and the error still occurs, you may need to replace the CD-Key. Follow the steps below to request a replacement CD-Key.

• If you get the message: "CD-Key in use by [another player]", then someone else may have used your Diablo 2 CD Keys to install the game. The [name] listed in this error message is not a Battle.net account name - it is the name entered when the game was installed. If this [name] is not the one you entered, please reinstall the game to ensure the correct key is entered. If the CD-Key is still in use, you may need a replacement key. Follow the steps below to request a replacement CD-Key.

Comments (10) Posted to interest 08/30/2008 Edit

Comcast adds 250 GB monthly cap

Comcast, the US largest broadband provider, is rolling out restrictions on its service usage, and subscribers whose use of the internet exceeds 250 GB of data a month will first get a warning call, and then on the second instance, their service will be suspended for a year…its current usage policy was amended online today, and this policy will start October 1, the company announced today. The more interesting part is that Comcast will NOT be provding any tools to monitor bandwidth usage, but has told users to search online for bandwidth monitoring tool, reports News.com. The FAQs about excess usage are here. Here’s how it justifies it: “250 GB/month is an extremely large amount of data, much more than a typical residential customer uses on a monthly basis. Currently, the median monthly data usage by our residential customers is approximately 2 - 3 GB. To put 250 GB of monthly usage in perspective, a customer would have to do any one of the following: — Send 50 million e-mails (at 0.05 KB/e-mail)– Download 62,500 songs (at 4 MB/song)– Download 125 standard-definition movies (at 2 GB/movie)– Upload 25,000 hi-resolution digital photos (at 10 MB/photo).” Of course HD streaming will also speed up that limit. This move from Comcast comes after its brush with FCC, where the regulator lambasted the company for blocking and slowing down P2P traffic on its service. Comcast insists this latest move has nothing to do with the FCC ruling against it. Source: Washington post

Comments (11) Posted to interest 08/30/2008 Edit

Subculture of Suspension Cell Lines

Subculture of Suspension Cell Lines

In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of lines subculture by dilution is relatively easy. But for lines that grow in clumps it may be necessary to bring the cells into a single cell suspension by centrifugation and resuspension by pipetting in a smaller volume before counting.

Schematic diagram of "Subculture of Suspension Cell Lines"

Materials

• Media– pre-warmed to 37oC (refer to the ECACC Cell Line Data Sheet for the correct medium)
• 70% Ethanol in water

Equipment

• Personal protective equipment (sterile gloves, laboratory coat, safety visor)
• Waterbath set to 37oC
• Microbiological safety cabinet at appropriate containment level
• Elisa plates
• Centrifuge
• CO2 incubator
• Inverted phase contrast microscope
• Haemocytometer
• Pre-labeled flasks & Cell Culture Plates(6 Well Plate, 24 Well Plate, 96 Well Plate)

Procedure

1. View cultures using an inverted phase contrast microscope. Cells growing in exponential growth phase should be bright, round and refractile. Hybridomas may be very sticky and require a gentle knock to the flask to detach the cells. EBV transformed cells can grow in very large clumps that are very difficult to count and the center of the large clumps may be non-viable.
2. Do not centrifuge to subculture unless the pH of the medium is acidic (phenol red = yellow) which indicates the cells have overgrown and may not recover. If this is so, centrifuge at 150g for 5 minutes, re-seed at a slightly higher cell density and add 10- 20% of conditioned medium (supernatant) to the fresh media.
3. Take a small sample of the cells from the cell suspension (100-200uL - Protocol 6 - Cell Quantification). Calculate cells/ml and re-seed the desired number of cells into freshly prepared flasks without centrifugation just by diluting the cells. The data sheet will give the recommended seeding densities.
4. Repeat this every 2-3 days.

Key Points

1. If the cell line is a hybridoma or other cell line that produces a substance (e.g. recombinant protein or growth factor) of interest retain the spent media for analysis.

Source: Sigma-Aldrich

Comments (1) Posted to interest 08/30/2008 Edit

Apple iTunes blocked in China

Users of Apple’s music store in China have been reporting problems downloading music since the beginning of the week. Chinese authorities have not directly confirmed blocking iTunes. Requests for comment have gone unanswered, or reporters have been told officials had no information on the block. However, the timing of this latest blockage seems to coincide with the release of a pro-Tibet album by the Art of Peace Foundation, which was released on Sunday. Users across several threads in Apple’s support forums are reporting issues. The first reports came in Monday, and seem to be widespread.

Forty Olympic athletes reportedly downloaded the album in solidarity with the group, with some even downloading it once they arrived in Beijing, the Foundation said. Although Chinese iPod and iPhone owners have had access to iTunes, it doesn’t actually provide them with music, though it does provide iPhone users with apps. So users often find themselves logging onto overseas stores or even other services in order to download music. Apple did confirm that some were having problems with access within China, but declined to comment further. Users reportedly complained on Apple sites in China that the ban hurt users more than it did iTunes.

Source: Betanews

Comments (0) Posted to interest 08/26/2008 Edit

iPhone Jailbreak Now Quickpwned

For all of you that care, or have/getting an iPhone - iPhone Jailbreak Now Quickpwned: No Need for iTunes-Based Restore

News is just out of a new way to jailbreak your iPhone, and this time the tool is specifically designed to work “quickly and easily, without requiring a full restore.” Quickpwn has been released as a beta, intended to complement the main PwnageTool. It works with iPhones and iPod’s running 2.0.1 firmware, and operates very quickly without needing iTunes to rebuild/restore your device afterwards. This beta version is basically feature-complete, but lacks a “funky UI” (they’re adding that soon) and is currently windows only (other OS’s are “coming soon”). It’s available here. Keep your eyes open for a complete version (though remember that even though it’s development software, it’s apparently “impossible for this to permanently damage your device”).

Link to Windows Version of Pwnage Tool: Rapidshare

Comments (0) Posted to interest 08/20/2008 Edit